BCA Assay for Protein Determination
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BCA Assay for Protein Determination
Purpose The Bicinchoninic Assay (BCA) is a contemporary method for determining protein concentration. It is based off of the Biuret reaction which involves the coordination between the peptide...
Oct 6, 2017
Bicinchoninic Acid Assay Principle
Protein Assay Methods with Principle, Advantages, Uses
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You must make 1 l OF 0.2 m acetic acid (CH3COOH). All you have available is concentrated glacial acetic acid (assay value, 98%; specific gravity, 1.05 g/mL). It will take how many milliliters of acetic acid to make this solution. Assume a gram molecular weight of 60.05 grams.
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